Contestants in LOL: Last One Laughing UK 2025 use a variety of comedic styles and strategies to make others laugh while keeping a straight face themselves. These include deadpan humor, surreal comedy, improvisation, and character-driven sketches. For example, Bob Mortimer is known for his surreal comedy, while Jimmy Carr is recognized for his dark and deadpan humor. The comedians employ these diverse approaches to try to break their opponents' composure during the competition.

The main contestants in LOL: Last One Laughing UK 2025 include well-known UK comedians such as Richard Ayoade, Daisy May Cooper, Joe Wilkinson, Bob Mortimer, Roisin Conaty, Sara Pascoe, Lou Sanders, Joe Lycett, Rob Beckett, Harriet Kemsley, and Judi Love. The show is hosted by Jimmy Carr, with Roisin Conaty also serving as a host. Special guests like Danny Dyer and Nick Mohammed appear in some episodes.

The interaction between contestants is central to the comedic content of LOL: Last One Laughing UK 2025. The comedians engage in direct challenges, improvisational exchanges, and attempts to provoke laughter from each other while maintaining their own composure. This dynamic creates spontaneous humor and showcases each comedian's unique style, contributing to the show's unpredictable and entertaining atmosphere.

Jimmy Carr serves as the host of LOL: Last One Laughing UK 2025, guiding the competition and setting the tone with his characteristic dark and deadpan humor. He introduces challenges, enforces rules, and interacts with contestants, adding an additional layer of comedy through his hosting style. His presence helps frame the comedic narrative and maintains the show's pace and structure.

Recurring comedic themes in LOL: Last One Laughing UK 2025 include the tension between maintaining a straight face and the absurdity of the comedians' attempts to make each other laugh. Themes of competition, improvisation, and the use of personal comedic trademarks (such as Bob Mortimer's surrealism or Jimmy Carr's deadpan delivery) appear throughout the episodes. The show also often features character sketches and playful mockery among contestants, creating a layered comedic experience.